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HSTO104 — Embedding, Microtomy, and Staining

3 credits · 3 hours

HSTO 104 - Embedding, Microtomy, and Staining HSTO 104 - Embedding, Microtomy, and Staining 3 Credit(s). Explains the embedding of tissue processed by paraffin, factors in the choice of paraffin, melting points and influence on support. Students review the ultrastructure of the cell and the differences between nuclear and cytoplasmic staining. In addition, topics include theory of staining and impregnation, compassion of natural and synthetic dyes, examine types and uses for hematoxylin and differentiation between progression, regressive and polychromatic staining. Troubleshooting issues with Hematoxylin and Eosin (H&E) staining and contrast resinous media versus aqueous media will be explored. Course once a year (fall or spring). Prerequisite(s): HSTO 101 and HSTO 102 Common Course Outline: HSTO 104 CCO

Prerequisites: HSTO101, HSTO102

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