BIOTECH 220 — Cell Culture Laboratory
Covers the basic techniques of plant and animal cell culture. Plant unit includes media preparation isolation of explants and establishment of callus from suspension cultures, growth factor bioassays, regeneration of whole plants from tissue and plant genetic engineering techniques. Mammalian cell units include media preparation, maintenance of cultured cells, transfection of cultured cells, cloning and ELISA assays and monoclonal antibody production. Writing assignments, as appropriate to the discipline, are part of the course.