BCH281L — Biochemistry Lab
This laboratory course is designed to be taken concurrently with BCH 280 and is a project-based course that models biochemistry research. Course involves the following: 1. Purification of enzyme from natural sources utilizing high-speed centrifugation, IEX, and affinity chromatography. 2. Characterization of enzyme by gel electrophoresis, Bradford assay, and specific substrate assay. 3. Analysis of enzyme function by kinetic study. (Spring Semester) Course Learning
Prerequisites: CHMY221