BIOT 224 — Gene Therapy Fundamentals
Introduces students to the gene therapy field and the fundamentals of viral vectors. The overview of gene therapy drug development process, types of viral vectors and their components, and viral production and purification will be taught. Hands-on training for techniques used in the gene therapy industry including cell and virus banking, plasmid isolation, transfection and transduction, Tangential Flow Filtration, digital PCR, and ion exchange chromatography using AKTA go system will be taught. The course may include one or more guest lectures.