BIOT230 — Applied Immunology
Brief survey of the critical, biotechnology relevant components of the innate and specific immune system including immune cells, cytokines, and antibodies. A survey of the related signal transduction systems such as JAK STAT and NfκB pathways. A brief description of immunotherapies such as Dendritic cell vaccines, CAR-T, immune checkpoint therapies monoclonal antibodies, and alternate antibody formats such as BiTEs and DARTs. Antigen antibody interactions, epitope mapping, and measurement of binding affinity. Principles of immunoassays with an emphasis on Western Blotting, ELISAs and Immunohistochemistry. Practical aspects such antibody labeling, antibody handling, storage and dilution, analysis of immunoassay data . Three hours lecture. 3 semester hours Course
Prerequisites: BIOT200