BIOT231 — Immunological Methods
(G only) Brief survey of the most common immunoassay techniques used to detect biomarkers qualitatively and quantitatively. Sample preparation and buffers for immunoassays. Handling of antibodies such as reconstitution and dilution of primary and secondary antibodies. Determination of antibody specificity. Use of multichannel pipettes, multiwell plates and plate readers. Western blotting, ELISA and immunohistochemistry. Bench top purification of polyclonal or monoclonal antibodies using protein A chromatography. Preparation of an alum absorbed immunogen. PRE- or Three hours laboratory each week. 1 semester hour Course
Prerequisites: BIOT201