BIOT251 — Techniques of Biomanufacturing
(G only) Introduction to the process of producing a biological product using a cell line. The course will be organized as a production campaign in a simulated cGMP environment. Students will complete a batch record as they produce a biological product. Emphasis will be on upstream and downstream processes. Preparation of media and buffers. The upstream component begins with a vial thaw and flask seeding. Closed system scale-up from a shake flask to a 3-liter closed system single use bioreactor. Probe assembly and calibration. TFF separation of proteins. Use of FPLC protein purification systems including associated software. SDS-PAGE analysis of the purified protein. PRE- or Three hours laboratory each week. 1 semester hour Course
Prerequisites: BIOT121, BIOT201