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BIOT120 — Introduction to Cell Culture

An introduction to the basic principles of cell culture. Classification and origin of cells in culture, growth curves and associated calculations, cell culture laboratory equipment, causes of contamination, detection of contamination, environmental monitoring, prevention of contamination, cell line authentication. Cell culture growth environment incubators, atmosphere, buffers, media and media supplements. Experimental design with an emphasis on root cause analysis. Assessment Level(s): ENGL 101 / ENGL 011 , MATH 050 . Two hours lecture. 2 semester hours May not be taken concurrently with MATH 017 or MATH 020 or MATH 030 or MATH 045 without appropriate Math assessment score. Course

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