BIOT200 — Protein Biotechnology
An introduction to protein structure and function. Primary, secondary, tertiary, and quaternary structure are discussed in relation to protein function and stability. Causes of protein denaturation and its prevention by formulations for biologics. Functional assays for proteins such as enzymes and receptors are described in terms of experimental design, data collection, and data analysis. Strategies and methods of protein purification and quality determination such as chromatographic, electrophoretic and spectrophotometric techniques, peptide mapping and sequencing are presented. Relevant calculations such as buffer and solution preparation, standard curves, and kinetic assays will be discussed. Three hours lecture. 3 semester hours Course
Prerequisites: BIOL150, CHEM131