BIOT201 — Protein Biotechnology Skills
(G only) Provides an introduction to the skills needed to assess protein structure and function, such as protein quantitation assays and standard curves. Solution and buffer preparation, micropipetting, and use of standard equipment such as centrifuges and spectrophotometers. SDS-PAGE, staining, and documentation of gels. Strategies and methods of protein purification are considered along with emphasis on chromatographic and electrophoretic techniques. An emphasis on peptide mapping and sequencing is included. PRE- or Three hours laboratory each week. 1 semester hour Course